Neuroprotective Activity of ()-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity

Figure 6

Effect of EGCG on LPS-induced production of ROS in neurons. Primary human neuronal cultures were treated with or without LPS at indicated concentrations for 48 h (a) or with indicated concentrations of EGCG for 1 h prior to 100 ng/mL of LPS treatment for additional 48 h (b). The ROS production was examined by labelling cells with a cell-permeable nonfluorescent probe 2′,7′-dichlorofluorescin diacetate and the fluorescence was measured by a fluorescence microplate reader with excitation at 488 nm and emission at 527 nm. (c) The expression of iNOS in neurons treated with LPS in the presence of indicated concentration of EGCG. Data were expressed as mean ± SD of three independent experiments. , as compared with control; , as compared with LPS treated.

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(b)

(c)