Tolerogenic Dendritic Cells Generated with Tofacitinib Ameliorate Experimental Autoimmune Encephalomyelitis through Modulation of Th17/Treg Balance

Figure 4

Adoptive transfer of Tofa-treated DCs decreased the proportion of IL-17+ and IFN-γ+ T cells and induced expansion of CD25+Foxp3+ Tregs from EAE mice. CD4+ T cells were sorted from spleens of EAE mice on day 23 after immunization, IL-17, IFN-γ (a), and Foxp3 (b) were measured by intracellular cytokine staining, and a set of representative data of eight mice per group was presented. Histograms showed the percentage of IL-17A+ (c), IFN-γ+ (d), and CD25+Foxp3+ cells (e). The values represent the means ± SEM, , and by ANOVA/Tukey’s post hoc test. The correlations between clinical scores (day 23) and the frequencies of IL-17-producing cells, IFN-r-producing cells and CD25+Foxp3+ cells in spleen of animal models (MOG pulsed mDCs with vehicle group, MOG unpulsed mDCs with 1 μM Tofa group, and MOG pulsed mDCs with 1 μM Tofa group) were presented as (f), (g), and (h), respectively, by using Spearmen’s rank test.

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