Tolerogenic Dendritic Cells Generated with Tofacitinib Ameliorate Experimental Autoimmune Encephalomyelitis through Modulation of Th17/Treg Balance

Figure 5

Antigen-specific T cell stimulation by pulsed-Tofa-DCs. (a) ImDC, mDCs, and Tofa-DCs were mixed with CFSE-labeled CD4+ T cells from EAE mice for 72 hours with different ratios of DCs : CD4+ T (1 : 10, 1 : 30, and 1 : 100), and the proliferation of T cells was determined by CFSE dilution through flow cytometry. (b) Graphic shows the average ± SEM of the percentage of proliferating CFSE-T cells. The representative results of three independent experiments with similar findings are shown. Statistical testing was performed using the Mann-Whitney test. and .

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