Tolerogenic Dendritic Cells Generated with Tofacitinib Ameliorate Experimental Autoimmune Encephalomyelitis through Modulation of Th17/Treg Balance

Figure 6

pulsed-Tofa-DC induced the hyporesponsiveness of antigen-specific T cells. (a) Activated CD4+ T cells were harvested and the expressions of IL-17A, IFN-γ, and foxp3 were analyzed by intracellular staining and flow cytometry in the gate of CD4+ T cells or CD4+ CD25+ T cells. (b) Histograms showed the percentage of IL-17A, IFN-γ producing cells, or CD25+Foxp3+ T cells in coculture experiments under different conditions. The representative results of three independent experiments with similar findings are shown. Statistical testing was performed using the Mann-Whitney test. Values represent the means ± SEM, , and .

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