Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance

Table 4

Sensitivity observed for each assay in the presence of 0, 20, and 100 µg/mL of Novimab. The sensitivity was calculated as the lowest PC concentration where the signal obtained was above the threshold value.

	Sensitivity (ng/mL of reference ADA)
Technology used	ELISA		MSD		Gyrolab		AlphaLISA
Acid dissociation step	No	Yes	No	Yes	No	Yes	No	Yes
Novimab concentration (μg/mL)
0	5	100	2	20	2	5	20	20
20	1000	100	250	50	100	5	10000	1000
100	10000	250	1000	50	500	50	>10000	10000