Research Article

Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines

Table 1

Immune effects at various time points after initiation of stimulation.

StimulusRead-out
CD40CD69CD70CD80CD83CD86MHC IMHC IIIL6IL8ProliferationIgGIgM
24 hours2 days3 days7 days

TNP-Ficoll+++++++++++++
TNP-BSA+++++++++++++++++++++
Anti-IgM++++++++++++++NA
Anti-IgM
+ IL4
++++++++++++++++++++++NA
Anti-CD40NA++++++++++++++++
Anti-CD40
+ IL4
NA++++++++++++++++++++++++++
Anti-CD40
+ IL21
NA++++++++++++++++++++++++
IL21+++++++++++++++++
LPS++++++++++++++++
Resiquimod+++++++++++++++++++++++++++
ODN2006++++++++++++++++++++++++++++++++++
Pansorbin
+ IL2 + IL10
++++++++++++++++++++++++++++++++
Pokeweed+++++++++++++++++++

In four independent experiments, different in vitro stimulatory conditions were tested on freshly isolated human B cells; the strength of their effect on proliferation, Ig and cytokine production, and expression of cell surface markers is represented in the table (+++ = strong effect, ++ = moderate effect, + = weak effect, and NA = not analysed). For the definition of weak, moderate, or strong effect, see Section 2.