Immunomodulatory Effects of 1,25-Dihydroxyvitamin D₃ on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens

Figure 4

Viability, recovery, and phenotypic characteristics of conventional and 1,25(OH)₂D₃-treated iDC from healthy controls before and after cryopreservation. CD14+ monocytes were cultured for 7 days in the presence of IL-4 and GM-CSF or in the presence of IL-4, GM-CSF, and 1,25(OH)₂D₃ to obtain immature conventional DC or 1,25(OH)₂D₃-treated DC, respectively. On day 7, iDC were frozen (i.e., precryo iDC). Following a 2 h resting phase after thawing, iDC were left untreated for 24 h (i.e., postcryo iDC). (a) Viability of conventional and 1,25(OH)₂D₃-treated iDC of healthy controls () was determined on day 7 of DC culture (i.e., precryo) and 26 h after thawing (i.e., postcryo). Recovery is expressed as the ratio of cells harvested before and after cryopreservation. (b) The MFI of (A) CD86, (B) CD80, (C) CD83, and (D) HLA-DR by immature conventional DC (open bars) or 1,25(OH)₂D₃-treated DC (black bars) of healthy controls () was determined. Results are expressed as mean ± SEM. MFI, mean fluorescence intensity, and iDC, immature DC.

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