Immunomodulatory Effects of 1,25-Dihydroxyvitamin D₃ on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens

Figure 5

Allogeneic T cell-stimulatory capacity of 1,25(OH)₂D₃-treated DC before and after cryopreservation. Fresh and frozen iDC and mDC were cocultured with allogeneic responder PBL at a 1 : 10 ratio. Nonstimulated PBL served as negative control, while allogeneic responder cells stimulated by mitomycin C-treated PBL (10 : 1 responder/stimulator ratio) were used as positive control. After 6 days, cell culture supernatant was collected and the secreted level of IFN-γ was used as a measure for allostimulatory capacity by means of IFN-γ ELISA. Each condition was measured in triplicate. Results of healthy controls () are expressed as mean ± SEM. The open bars and black bars represent the measured IFN-γ secretion before cryopreservation and after cryopreservation, respectively. iDC, immature DC; cc-mDC, cytokine cocktail-matured DC; and PBL, peripheral blood lymphocytes.