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Journal of Immunology Research
Volume 2016, Article ID 5392623, 16 pages
http://dx.doi.org/10.1155/2016/5392623
Research Article

Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens

1Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Antwerp, Belgium
2Department of Neurology, Antwerp University Hospital, 2650 Edegem, Belgium
3Department of Neurology, Born Bunge Institute, Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Antwerp, Belgium
4Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Antwerp, Belgium
5Division of Immunology, Germans Trias i Pujol University Hospital and Research Institute, Campus Can Ruti and Department of Cell Biology, Physiology and Immunology, The Autonomous University of Barcelona, 08913 Bellaterra, Spain
6Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 2650 Edegem, Belgium

Received 25 February 2016; Revised 6 June 2016; Accepted 27 June 2016

Academic Editor: Moisés E. Bauer

Copyright © 2016 Wai-Ping Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: Phenotypic characteristics of in vitro differentiated iDC and cc-mDC from healthy controls and MS patients CD14+ monocytes were cultured for 6 days in the presence of IL-4 and GM-CSF or in the presence of IL-4, GM-CSF and 1,25(OH)2D3 to obtain conventional iDC (open dots) or 1,25(OH)2D3-treated iDC (filled triangles) respectively. On day 6, DC were stimulated with a cocktail of pro-inflammatory cytokines (i.e. cc-mDC) or left untreated (i.e. iDC). The mean fluorescence intensity (MFI) of (A) PD-L1, (B) ILT-3, (C) DC-SIGN, (D) CD14 and (E) CCR7 by DC of healthy controls (n=7) and MS patients (n=10) is determined by flow cytometry. Horizontal lines show the mean. Abbreviations used: MFI, mean fluorescence intensity; iDC, immature DC; cc-mDC, cytokine cocktail-matured DC.

  1. Supplementary Material