Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation

Figure 1

No significant differences between the non-GVHD and GVHD groups regarding major lymphocyte subsets or T-cell maturation subsets in unmanipulated donor samples. Flow cytometry-acquired phenotypic data analysed in blood samples from donors. The data were divided into two groups based on if patients did or did not develop acute GVHD grades II–IV. Each dot represents the cell-subset frequency of one donor and horizontal bars indicate the median of each group. Representative FACS plots are shown below each dot-plot of one non-GVHD and one GVHD patient. (a) Percentages of total T-cells (CD3⁺), NK-cells (CD3⁻CD56⁺), and B-cells (CD3⁻CD19⁺). No differences were observed for these cellular subsets between the non-GVHD and GVHD patient groups. (b) Proportions of T-cell subsets at different maturation states in the total T-cell population, expressed as median percentages. Terminal, terminally differentiated T-cells (CD45RO⁻CCR7⁻); effector, effector memory T-cells (CD45RO⁺CCR7⁻); central, central memory T-cells (CD45RO⁺CCR7⁺); naïve, naïve T-cells (CD45RO⁻CCR7⁺). No differences were observed.

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