Biological Character of RetroNectin Activated Cytokine-Induced Killer Cells

Figure 1

RetroNectin activated CIK cells had stronger proliferative ability. (a) Proliferative curve of OKT-CIK and R-CIK cells. The proliferative speed of R-CIK cells was much higher than that of OKT-CIK cells, . (b) Mean percentage of OKT-CIK and R-CIK cells undergoing early apoptosis (Annexin⁺PI⁻) and late apoptosis/necrosis (Annexin⁺PI⁺). for the comparison, . (c) Continual proliferative curve of OKT-CIK and R-CIK cells in medium without IL-2. R-CIK cells could continue expanding 4 days after IL-2 was withdrawn from the medium, and the maximum average amplification is 6 times. OKT-CIK cells could only continue expanding 2 days in the same condition, and the maximum average amplification is 3 times, . (d) Shape of cultured OKT-CIK and R-CIK cells (400x).

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