The Generation and Characterization of Recombinant Protein and Antibodies of Clostridium perfringens Beta2 Toxin

Figure 4

Generation of hybridoma cell lines producing antibodies against CPB. Ten predicted antigenic epitopes were used for generation and screening of antibodies against CPB2 using a SEAL technology (Surface Epitope Antibody Library) developed by Abmart (Shanghai, China). (a) A graph showed an overall structure of protein of interest and the 10 epitopes selected peptide for immunization to generate hybridoma cell lines producing specific antibodies against CPB2. The value of -axis indicated locations of amino acids (AAs) from N-terminal to C-terminal of CPB2 protein; the value of -axis indicated scores of algorithm, a high score represented an epitope with high possibility. The ten selected epitopes (1–10) were highlighted with blue on the graph. The epitopes 5 and 10 were circled in red, and antibodies to epitopes 5 and 10 were further identified to react with rCPB2 in this study. (b) The IDs, AA regions, AA sequence, and hybridoma cell clones of respective epitopes examined in this study. The peptide sequences in italic were able to generate cell clones producing antibodies that were identified to react with rCPB2 (clones 1E23, 2G7, and 2H7, labeled in red and italic).

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