Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles

Figure 1

Phenotyping of the different subsets of leukocyte-derived sEVs. The different subsets of leukocyte-derived sEVs were phenotyped for a panel of selected markers using the EV Array. The sEVs were captured by antibodies targeting the selected markers and subsequently detected by addition of an antibody cocktail against CD9, CD63, and CD81. The heat maps illustrate the results from each of the three independent experiments divided into lineage-specific markers (a), vesicle-related markers (b), and general immunological markers (c) and present the relative intensities of each of the markers as indicated by the colored bars. Data are presented as the mean value of the triplicates.