Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles

Figure 3

Cellular expression of CD9, CD63, and CD81 on five leukocyte subpopulations. The cellular expression pattern of CD9, CD63, and CD81 was investigated for leukocytes present in freshly drawn whole blood (a) as well as for isolated subpopulations of leukocytes following two days of culture (b). For each of the subpopulations, the expression pattern was determined by flow cytometry. The expression from two representative donors is illustrated in histograms. Isotype controls were included for every leukocyte subpopulation and when the obtained signals were similar, only one isotype control is depicted. Regarding the cultivated cells, a representative lymphocyte isotype control is shown. Due to differences in the level of the isotype control between the lymphocytes and the monocytes, the monocyte isotype control is also shown. The interdonor variation in the expression levels of the different markers is displayed next to each histogram. Data are presented as median fluorescence intensities (MFI) with min and max indicated in parenthesis from the ten (whole blood) or three donors (cultivated cells), respectively.

(a)

(b)