Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles

Figure 4

Summary of results on the presence of selected lineage-specific and vesicle-related markers on five leukocyte subpopulations and the corresponding leukocyte-derived sEVs. The expression of selected lineage-specific and vesicle-related markers was determined for freshly isolated leukocytes and for cultured leukocyte subpopulations. The cellular expression was determined by flow cytometry. The level of the different markers was grouped into negative (−), low (+), intermediate (++), or high (+++) corresponding to the following signal intensities: <100, 100–1000, 1000–10000, and 10000–100000, respectively. The results presented on freshly isolated leukocytes and on cultured leukocytes are based on median values of ten or three individual experiments, respectively. The EV Array determined the presence of the markers on the corresponding leukocyte-derived sEVs. The level of the different markers is grouped into negative (−), low (+), intermediate (++), or high (+++) corresponding to the following signal intensities: 0–0.1, 0.1–1, 1–2.5, and 2.5–5.5, respectively. The results presented on the leukocyte-derived sEVs are based on mean values of three individual experiments. Ag: antigen; Ab: antibody; APC: antigen-presenting cell.