Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles

Table 1

Overview of the markers selected for phenotyping of sEVs.

Immunological markers				Vesicle-related markers
Functional	Regulatory	Adhesion	Lineage-specific and others	Tetraspanins	Others
HLA-DR	CTLA-4	CD11a	CD3	CD9	TSG101
HLA-ABC	PD-L1	ICAM-1	CD4	CD63	Alix
CD28			CD8	CD81	Flotillin-1
CD80			CD14	CD82	Annexin V
CD49d			CD16
TLR3			CD19
Fas-L			CD37
MIC A/B			CD41
TNF RI			CD42a
TNF RII			CD45
			CD56
			CD83

HLA: human leukocyte antigen; TLR: Toll-like receptor; TNF: tumor necrosis factor: RI: receptor I, RII: receptor II; MIC A/B: major histocompatibility complex class I-related chain A/B; CTLA-4: cytotoxic T-lymphocyte associated protein-4; PD-L1: programmed death-ligand 1; ICAM-1: intercellular adhesion molecule 1; TSG101: tumor susceptibility gene 101.