Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis

Figure 2

Effect of previous PG inoculation on cytokine production by spleen cells from arthritic mice. (a) IFN-γ, (b) IL-17, (c) IL-6, (d) IL-5, and (e) IL-10 production by spleen cells assessed by enzyme linked immunosorbent assay. Control (−): healthy group not previously injected with PG; control (+): arthritic group not previously injected with PG; 2xPG50, 2xPG100, and 3xPG50: arthritic groups previously injected with two (50 μg), two (100 μg), or three (50 μg) PG doses, respectively. Six to ten animals per group from one representative experiment of two performed. compared with the unstimulated counterpart and compared with the control (+) group.

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