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Journal of Immunology Research
Volume 2016 (2016), Article ID 7249369, 9 pages
http://dx.doi.org/10.1155/2016/7249369
Research Article

Antigen-Specific IFN-γ Responses Correlate with the Activity of M. tuberculosis Infection but Are Not Associated with the Severity of Tuberculosis Disease

1Department of Immunology, Central Tuberculosis Research Institute, Yauza Alley 2, Moscow 107564, Russia
2Phthisiology Department, Central Tuberculosis Research Institute, Yauza Alley 2, Moscow 107564, Russia
3Department of Microbiology, Central Tuberculosis Research Institute, Yauza Alley 2, Moscow 107564, Russia

Received 3 June 2016; Revised 9 September 2016; Accepted 21 September 2016

Academic Editor: Frederick D. Quinn

Copyright © 2016 Irina Yu. Nikitina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: The levels of IFN-γ secretion measured in QFT assay in patients having different severity of TB disease. Patients were grouped as based on the characteristics of TB disease, as described in Figure 2. Each TB characteristic was scored as described in the section 3.1. The levels of IFN-γ were determined using QFT assay. The values of IFN-γ in MIT samples greater than 10 IU/mL were assigned a value of 10 IU/mL, the upper limit of the standard curve in QFT. The exact levels of IFN-γ secretion in AG tubes were determined (samples from patients having high levels of IFN-γ in AG tubes were diluted and QFT was repeated for all probes, i.e., NIL, AG, and MIT).

Figures on X-axis indicate scores. In ANOVA, the differences between the groups were insignificant. Shown are the differences determined in Mann-Whitney test (*, p<0.05; **, p<0.01; ***, p<0.001).

Supplementary Figure 2: The performance of QFT in HD, TBC, and patients with different TB activity. The levels of IFN-γ were determined using QFT assay. The values of IFN-γ in MIT samples greater than 10 IU/mL were assigned a value of 10 IU/mL, the upper limit of the standard curve in QFT. The exact levels of IFN-γ secretion in AG tubes were determined (samples from patients having high levels of IFN-γ in AG tubes were diluted and QFT was repeated for all probes, i.e., NIL, AG, and MIT). For other details, see footnote to Figure 3.

  1. Supplementary Material