Lactobacillus rhamnosus GG Activation of Dendritic Cells and Neutrophils Depends on the Dose and Time of Exposure

Table 1

Surface marker expression on DC after direct and indirect exposure via neutrophils to BCG and LGG.

Marker	DC	Percentage of CD11c^+ cells
		Control	5 : 1 (BCG)	5 : 1 (LGG)	10 : 1 (LGG)	100 : 1 (LGG)
CD40	2 h	6.0 ± 3.3	22.5 ± 3.3	32.1 ± 4.9	32.7 ± 0.3	50.7 ± 2.6
	18 h	2.2 ± 0.5	19.3 ± 5.4	20.5 ± 6.9	19.0 ± 6.8	7.0 ± 0.8
	+neutrophils (2 h)	2.2 ± 0.4	27.8 ± 3.5	22.9 ± 5.2	25.4 ± 8.7	10.4 ± 2.0
CD80	2 h	9.9 ± 1.4	39.3 ± 10.4	18.2 ± 0.8	18.1 ± 3.3	34.0 ± 4.2
	18 h	9.6 ± 1.6	30.7 ± 7.5	17.9 ± 0.5	19.7 ± 2.5	19.6 ± 0.8
	+neutrophils (2 h)	6.1 ± 1.6	33.8 ± 8.9	21.2 ± 5.0	16.6 ± 7.6	16.1 ± 7.8
CD83	2 h	6.5 ± 0.2	29.6 ± 5.7	38.4 ± 3.7	32.7 ± 1.3	36.2 ± 4.7
	18 h	5.1 ± 1.5	27.5 ± 10.5	8.4 ± 1.9	4.8 ± 0.8	6.5 ± 1.4
	+neutrophils (2 h)	3.6 ± 0.2	31.2 ± 4.8	9.8 ± 1.3	8.0 ± 0.8	11.1 ± 2.8
CD86	2 h	17.0 ± 0.3	72.0 ± 5.4	37.1 ± 8.1	41.6 ± 1.4	58.5 ± 6.1
	18 h	14.8 ± 1.9	71.0 ± 7.9	57.6 ± 0.1	56.5 ± 1.3	29.7 ± 6.0
	+neutrophils (2 h)	13.7 ± 0.2	68.0 ± 4.8	68.3 ± 6.4	76.5 ± 4.4	54.9 ± 3.8
MHC; class II	2 h LGG	31.7 ± 1.8	61.9 ± 2.5	69.9 ± 8.8	70.7 ± 12.8	77.8 ± 11.1
	18 h LGG	32.6 ± 2.7	59.2 ± 6.1	58.4 ± 12.5	52.9 ± 7.2	57.4 ± 11.6
	+neutrophils (2 h)	34.7 ± 10.1	55.3 ± 10.2	58.5 ± 12.9	52.1 ± 6.3	64.0 ± 9.4

⁢ compared to groups treated with 5 : 1 and 10 : 1 LGG, respectively.
Data are presented as the mean ± SEM.