Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver

Table 3

Parameters of cachexia, liver weight, and severity of arthritis (hind paw volume and arthritic score) in rats with adjuvant-induced arthritis on experimental days 14 and 28 treated with N-f-5HT and MTX.

Cachexia	CO	AA	AA-MTX	AA-N-f-5HT
Parameter
Severity of arthritis
Hind paw volume
Day 14	1.67 ± 0.01	1.82 ± 0.04	1.69 ± 0.03	1.87 ± 0.06
Day 21	1.78 ± 0.02	2.07 ± 0.08	1.72 ± 0.04	2.07 ± 0.05
Day 28	1.81 ± 0.02	1.98 ± 0.07	1.72 ± 0.03	1.95 ± 0.04
Arthritic score
Day 14	10 ± 0	15.1 ± 0.79	12.4 ± 0.72	16.7 ± 1.36
Day 21	11.25 ± 0.32	19.71 ± 1.11	14.4 ± 1.68	19.6 ± 1.37
Day 28	11.37 ± 0.26	22.29 ± 0.71	16.3 ± 1.11	19.5 ± 1.23
Cachexia
Body weight change (g)
Day 14	59.37 ± 1.80	33.25 ± 4.80	42.82 ± 3.74	39.92 ± 5.33
Day 21	75.28 ± 2.68	14.14 ± 4.97	31.15 ± 6.17	29.6 ± 4.52
Day 28	102.69 ± 4.32	28.1 ± 4.61	38.9 ± 8.03	55.32 ± 4.99
Liver weight (g)
Day 28	7.94 ± 0.17	7.99 ± 0.21	7.05 ± 0.23	8.32 ± 0.23

CO, control group; AA, adjuvant arthritis group; AA-N-f-5HT, adjuvant arthritis group given N-feruloylserotonin; AA-MTX, adjuvant arthritis group given methotrexate. The data represent the mean ± SEM; = 9-10. The symbols and + show significant difference: versus CO; versus CO; versus CO; versus AA; versus AA; versus AA.