Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation

Table 1

List of unique peptides and MRMs for ADA isotopes/subclasses used in the immunocapture-LC/MS assay.

Isotype/subclass	Unique peptide sequence	MRM pairs
Quantitation peptide
IgG1	GPSVFPLAPSSK	593.83→699.40
IgG2	GLPAPIEK	412.75→654.38
IgG3	WYVDGVEVHNAK	708.85→698.48
IgG4	GLPSSIEK	415.73→660.36
IgE	AEWEQK	395.69→590.29
IgM	GQPLSPEK	428.23→670.38
IgA1, IgA2	YLTWASR	448.73→620.32
Confirmation peptide
IgG1, IgG3	ALPAPIEK	419.76→654.38
IgG1, IgG3, IgG4	VVSVLTVLHQDWLNGK	603.34→1110.57
IgE	LEVTR	309.18→375.24
IgM	VSVFVPPR	450.77→615.36
IgA1, IgA2	VAAEDWK	409.71→648.30
IgA1	TFTCCAMTAAYPESK	688.31→765.38