Research Article
Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation
Table 2
LC/MS peak area ratio response and cut-points of ADA isotopes/subclasses in PEA negative human plasma with drug as ADA capture reagent.
| Plasma lot # | IgG1 | IgG2 | IgG3 | IG4 | IgE | IgM | IgA1 + IgA2 |
| 1 | 0.0277 | 0.0099 | — | 0.0107 | — | 0.1130 | 0.0041 | 2 | 0.0107 | 0.0008 | — | 0.0019 | — | — | 0.0059 | 3 | 0.0263 | — | — | 0.0012 | 0.0150 | 0.0084 | 0.0078 | 4 | 0.0093 | 0.0023 | — | 0.0053 | — | 0.0072 | 0.0057 | 5 | 0.0249 | 0.0244 | 0.0210 | 0.0099 | 0.0110 | 0.0474 | 0.0136 | 6 | 0.0060 | 0.0044 | 0.0110 | 0.0035 | — | 0.0326 | — | 7 | 0.0129 | — | — | 0.0037 | 0.0160 | 0.0159 | 0.0062 | 8 | 0.0637 | 0.0205 | 0.0570 | 0.0353 | 0.0140 | 0.0066 | 0.0174 | 9 | 0.0127 | 0.0008 | — | 0.0111 | — | 0.0035 | 0.0075 |
| Mean | 0.0216 | 0.0070 | 0.0099 | 0.0092 | 0.0061 | 0.0261 | 0.0086 | SD | 0.0177 | 0.0093 | 0.0192 | 0.0105 | 0.0074 | 0.0361 | 0.0043 |
| Cut-point (95%) | 0.0507 | 0.0224 | 0.0416 | 0.0265 | 0.0183 | 0.0854 | 0.0156 |
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—: no LC/MS response was detected.
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