Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation

Table 4

Calibration curve ranges and regression coefficients () of ADA isotype/subclasses using drug or mouse mAb as ADA capture reagent.

Isotype/subclass	Unique peptide	Calibration curve parameters
		Drug capture		mAb capture
		Range (µg/mL)		Range (µg/mL)
IgG1	GPSVFPLAPSSK	0.5–10	0.9919	0.5–10	0.9940
IgG2	GLPAPIEK	0.25–10	0.9939	0.1–10	0.9964
IgG3	WYVDGVEVHNAK	0.25–10	0.9858	0.25–10	0.9952
IgG4	GLPSSIEK	0.1–10	0.9919	0.25–10	0.9909
IgE	AEWEQK	0.1–10	0.9947	0.1–10	0.9976
IgM	GQPLSPEK	0.25–10	0.9988	0.25–10	0.9909
IgA1 + IgA2	YLTWASR	0.1–10	0.9974	0.1–10	0.9966