Bone Marrow Mesenchymal Stem Cells Enhance the Differentiation of Human Switched Memory B Lymphocytes into Plasma Cells in Serum-Free Medium
Effects of eosinophil and mesenchymal stem cells on the differentiation of switched memory B lymphocytes. Switched memory B lymphocytes were cultured as described in Figure 2(a) for the expansion and transition phases. Then, cells were exposed to L4.5 cells (L) alone or in combination with EOL-1 cells (E) or EOL-1 cells plus bone marrow MSC (M) during the differentiation phase. During that phase, all cultures were done in 21% and 8% O2 condition. (a) Plasma cell content was determined on day 20 by excluding debris and dead cells. Analysis relative to CD38+CD138+ cells is gated within the upper right (UR) quadrant, and results are shown in (b) and (d). Results for CD38hiCD138hi population, gated within R1 (red square), were analysed in (c) and (e). (g) Analysis for CD138hi cells gated within R2 (green square). (b) Frequencies of CD38+CD138+, (c) CD38hiCD138hi, (d-e) CD31+, (f) CD138+, and (g) CD138hi populations are shown for 7 independent experiments for cells cultured in the presence of L4.5 cells alone and for 5 independent experiments for LE and LEM assays. Statistical analyses were done using a Dunn multiple comparison -test with values as indicated: , , and . Data are presented as means ± SEM.
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