Bone Marrow Mesenchymal Stem Cells Enhance the Differentiation of Human Switched Memory B Lymphocytes into Plasma Cells in Serum-Free Medium
Cytokines and immunoglobulins secretions. All analyses were done at the end of the differentiation phase (day 20). (a and b) CXCL12 and TGF-β1 contents were determined in cell culture supernatants of differentiated cells. Control (Ctrl) corresponds to the supernatant of MSC cultured in α-MEM for 4 days in the absence of B lymphocytes. All other conditions correspond to B lymphocytes in coculture with MSC or L4.5 cells or B lymphocytes cultured in the presence of MSC which were seeded in the upper chamber of transwell (Trans). Cultures were done in parallel and at 21% or 8% O2. (c–g) IgG1, IgG2, IgG3, and IgG4 secretion were determined in cell culture supernatants on day 20. Statistical analyses were performed using a Dunn multiple comparison -test (, , and ). No significant differences were observed between MSC in direct contact and transwell assays at 8% O2 (; Kruskal-Wallis -test). Except for the results of MSC in direct contact, which were obtained from four independent experiments, all data are from five independent experiments and all are presented as means ± SEM.
We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.