Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma

Figure 2

Interference efficiency of p70S6K siRNA on mRNA and protein of p70S6K in EC9706 cells. (a) Expression of p70S6K mRNA. (b) Semiquantitative values of p70S6K mRNA normalized to GAPDH mRNA. (c) Expressions of p70S6K/p-p70S6K protein. (d) Semiquantitative values of p-p70S6K/p70S6K protein normalized to GAPDH. Results were from three independently repeated experiments analyzed by using Image J (NIH, USA); data were expressed as mean ± SD. , for mRNA results, and for protein expression, compared to those of EC9706 cells treated with negative control siRNA.

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