CD63 Promotes Hemocyte-Mediated Phagocytosis in the Clam, Paphia undulata

Table 1

Primer	Sequence ()	Comment
GR5P	CGACTGGAGCACGAGGACACTGA	Adaptor of 5′ RACE
GR5NP	GGACACTGACATGGACTGAAGGAGTA	Adaptor of 5′ RACE
GR3P	GCTGTCAACGATACGCTACGTAACG	Adaptor of 3′ RACE
GR3NP	CGCTACGTAACGGCATGACAGTG	Adaptor of 3′ RACE
CD63-R1	GATGGTCAGAAACAGGCCAAAGAAA	5′ RACE primer of First PCR
CD63-F1	GCAGACACTTCCACAGCGACGACAG	3′ RACE primer of First PCR
CD63-R2	CCGAAGCGAATAAACAAGCCCAGTCCGAAA	5′ RACE primer of Nest PCR
CD63-F2	TCATCTGGGACGCCACCCTTGGAAGTAC	3′ RACE primer of Nest PCR
CD63-QF	TAGATAAGGGATGTTACGAC	Real-time PCR for Pu-CD63
CD63-QR	TTCATGCTACACAAAATTAC	Real-time PCR for Pu-CD63
GAPDH-QF	ACTCGGAAAGGAGACCACCTACCA	Real-time PCR of GAPDH
GAPDH-QR	GAAACGACCTGATCCTCGGTGTAG	Real-time PCR of GAPDH
Luc-QF	GCTGGTGCCAACCCTATTCTCC	Real-time PCR of Luc
Luc-QR	AACCGCTTCCCCGACTTCCTTA	Real-time PCR of Luc
CD63-OF	ATGGGTTTTGTTTCGACACTCGCCAG	ORF check for Pu-CD63
CD63-OR	CTAAAAGTCTTTATGACCTGTCTTG	ORF check for Pu-CD63
RiCD63-F	TAATACGACTCACTATAGGGAGAGGGCTTGTTTATTCGCTTCGGGTCA	RNAi primer for CD63
RiCD63-R	TAATACGACTCACTATAGGGAGATAGTTGTTTGCTGTCGTCGCTGTGG	RNAi primer for CD63
RiLuc-F	TAATACGACTCACTATAGGGAGAGTCCGTTCGGTTGGCAGAAGCTATG	RNAi primer for Luc
RiLuc-R	TAATACGACTCACTATAGGGAGACATGCGAGAATCTCACGCAGGCAGT	RNAi primer for Luc

“F” indicates the forward primer; “R” indicates the reverse primer.