SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix

Figure 1

Principle of SEC assay. Fab488 is added to a serum sample. After incubation, Fab488 binds to the therapeutic mAb in the serum samples and to ADA complexes containing the therapeutic mAb (left). Formed complexes are separated by size exclusion chromatography (SEC) and the peaks quantified (right).