Rotavirus Recombinant VP6 Nanotubes Act as an Immunomodulator and Delivery Vehicle for Norovirus Virus-Like Particles

Figure 2

Internalization of VP6 nanotubes. Flow cytometry analysis of VP6 uptake by RAW 264.7 macrophages (a), JAWSII DCs (b), and human colorectal Caco-2 cells (c) after 24 h incubation in the presence of 100 μg/mL VP6. Cells exposed to rotavirus (RV) Wa and untreated cells incubated in culture medium only served as controls. VP6 was detected by intracellular staining with rabbit anti-rotavirus antibody followed by goat fluorescein isothiocyanate-conjugated anti-rabbit Ig antibody. Shown are overlaid histograms of cells exposed to VP6 nanotubes or RV Wa and untreated cells of one representative experiment of three independent experiments performed. Mean fluorescence intensity (MFI) values of each histogram are indicated.

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