CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction

Figure 3

Quantitative proteomic analysis CLAD liver allografts. iTRAQ quantitative proteomics analysis was performed using protein samples from BN-to-BN liver allografts (control) and BN-to-Lewis liver allografts (CLAD). The proteins were analyzed with 95% or greater confidence as determined by ProteinPilot Unused scores (≤1.3). The following parameters were applied: an unpaired -test with value < 0.05, a false discovery rate (FDR) of all peptide and protein identifications <1%, and max missed cleavages and an enrichment score = 2. There was a ≥1.50-fold change for upregulation or ≤0.67-fold change for downregulation. (a) GO term analyses were performed using the online tool from DAVID Bioinformatics Resources (version 6.7). Both gene numbers and values of specific gene groups were listed. (b) The top ranked 26 CLAD-associated proteins identified by functional annotation clustering/iTRAQ quantitative proteomic analysis. (c) The identified CLAD-associated proteins of liver allografts were categorized by subcellular distributions and functions based on PANTHER and Uniprot KB sources.