Overcoming the Constraints of Anti-HIV/CD89 Bispecific Antibodies That Limit Viral Inhibition

Figure 2

Immunoreaction of F240 × 14A8 Bi-Fab with gp41. Immunoreactivity of Bi-Fab antibody constructs with HIV antigen gp41. Antigen gp41 was coated at 2 µg/mL in PBS; the serial dilutions of Bi-Fab antibodies (Bi-Fab A, ●, and Bi-Fab B, ■) compared to that of serial dilutions of F240 antibody (▲); the reaction was developed by HRP-conjugated protein L (1 : 20,000) and measured by optical density at 490 nm. Results are representative of three different experiments and each experimental point is the mean ± standard deviation of triplicate wells.