Overcoming the Constraints of Anti-HIV/CD89 Bispecific Antibodies That Limit Viral Inhibition

Table 1

ADCVI activity of HIV-1 by F240 Bi-Fab antibody constructs.

	IC50 (g/mL)
	BaL	JR-FL	93MW960	SF162
	Clade B, R5	Clade B, R5	Clade C R5	Clade B, R5
Bi-Fab-A	3.38 ± 2.39	0.04 ± 0.04	27.50 ± 7.78	15.69 ± 5.49
Bi-Fab-B	4.43 ± 0.16	35.85 ± 5.87	1.93 ± 2.65	0.22 ± 0.04
Chemical bispecific	4.35 ± 1.2	NT	10.4 ± 7.7	10.7 ± 2.9

The ADCVI activity was determined by IC₅₀ that represents concentration (g/mL) of Bi-Fab antibody constructs required for 50% inhibition of HIV. Results are from three separate experiments.
The value reflects the comparison of Bi-Fab-A versus Bi-Fab-B.
Chemical conjugation of Fab fragments of F240 and 14A8 as described in [4].
NT: not tested.