Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral Cytotoxicity

Table 1

White opaque flat-bottom plates together with the Infinite M200 (Tecan) plate reader result in a robust signal-to-noise ratio.

Parameter	Experiment	Outcome
Plate opacity	White opaque versus transparent	Specific luciferase signals obtained from white opaque plates were 2-3-fold higher
Plate design	Flat-bottom versus V-shaped bottom	Only negligible well-to-well cross-talk was observed when flat-bottom plates were used
Plate reader	Wallac VICTOR2 (Perkin Elmer) versus Infinite M200 (Tecan) versus GENios Pro (Tecan)	Signals from the Infinite M200 device were 4-fold and signals from the GENios Pro 20-fold higher than those detected with the Wallac VICTOR2