Table 1: White opaque flat-bottom plates together with the Infinite M200 (Tecan) plate reader result in a robust signal-to-noise ratio.

ParameterExperimentOutcome

Plate opacityWhite opaque versus transparentSpecific luciferase signals obtained from white opaque plates were 2-3-fold higher

Plate designFlat-bottom versus V-shaped bottomOnly negligible well-to-well cross-talk was observed when flat-bottom plates were used

Plate readerWallac VICTOR2 (Perkin Elmer) versus Infinite M200 (Tecan) versus GENios Pro (Tecan)Signals from the Infinite M200 device were 4-fold and signals from the GENios Pro 20-fold higher than those detected with the Wallac VICTOR2