The Application of Dextran Sedimentation as an Initial Step in Neutrophil Purification Promotes Their Stimulation, due to the Presence of Monocytes

Figure 3

Dextran and Ficoll do not directly activate neutrophils. Blood was subjected to the dextran equivalent of 1-step high-density gradient centrifugation, and the Ficoll-equivalent of the 2-step method with erythrocyte sedimentation followed by low-density gradient centrifugation, after Ficoll 400 and dextran 266, were substituted for each other in the different purification media. Neutrophils purified by the 1-step method were assayed for chemotaxis and adherence (). Neutrophils purified from the 2-step sedimentation first method were assayed by adherence ().

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