The Application of Dextran Sedimentation as an Initial Step in Neutrophil Purification Promotes Their Stimulation, due to the Presence of Monocytes
Dextran and Ficoll do not directly activate neutrophils. Blood was subjected to the dextran equivalent of 1-step high-density gradient centrifugation, and the Ficoll-equivalent of the 2-step method with erythrocyte sedimentation followed by low-density gradient centrifugation, after Ficoll 400 and dextran 266, were substituted for each other in the different purification media. Neutrophils purified by the 1-step method were assayed for chemotaxis and adherence (). Neutrophils purified from the 2-step sedimentation first method were assayed by adherence ().
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.