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Journal of Immunology Research
Volume 2017 (2017), Article ID 1273940, 7 pages
https://doi.org/10.1155/2017/1273940
Research Article

Low Dose of Lipopolysaccharide Pretreatment Preventing Subsequent Endotoxin-Induced Uveitis Is Associated with PI3K/AKT Pathway

Department of Ophthalmology, Beijing Chao Yang Hospital, Capital Medical University, No. 8 South Gongren Tiyuchang Road, Chao Yang District, Beijing 100020, China

Correspondence should be addressed to Hong Lu; moc.kooltuo@umculgnoh

Received 20 January 2017; Revised 29 May 2017; Accepted 1 June 2017; Published 18 July 2017

Academic Editor: Mario Clerici

Copyright © 2017 Nan Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. To explore the effects of LPS pretreatment on endotoxin-induced uveitis and PI3K/AKT pathway. Methods. Endotoxin-induced uveitis was induced by a single subcutaneous injection of 200 μg LPS. For the endotoxin tolerance group, induction of EIU was preceded by daily subcutaneous injection of 0.1 mg/kg LPS for five days. Clinical scores were graded at 24 h after EIU under a slit lamp microscope. HE stain was performed to observe the histopathology. Aqueous humor TNF-α concentration was quantified with enzyme-linked immunosorbent assay. The expressions of PI3K and AKT were detected through Western blot analyses, and the activation of AKT was detected through immunofluorescence study. Results. Endotoxin tolerance produced suppressive effects by significantly reducing the inflammatory reaction of anterior segment of the rats as measured by slit lamp and histopathology. Low dose of LPS pretreatment significantly reduced TNF-α concentrations and the expressions of PI3K and AKT. Furthermore, the activation of AKT was also inhibited. Conclusions. LPS pretreatment can ameliorate endotoxin-induced uveitis in rats. This protection of endotoxin tolerance against EIU is associated with PI3K/AKT pathway by reducing level of TNF-α in the aqueous humor.