Intracellular pH Regulates TRAIL-Induced Apoptosis and Necroptosis in Endothelial Cells

<div>MVEC death at acidic condition is dependent on PARP-1. (a) MVECs (triplicates) were treated with 100 ng/ml TRAIL, 100 nM SMC, 50 <i>μ</i>M zIETD-fmk, and/or 3-ABA at pH 7.4. Kinetic cell death responses were measured by Sytox green staining and quantified by IncuCyte live-cell imager. (b) MVECs were treated with TRAIL, SMC, zIETD-fmk, and/or 3-ABA at pH 6.7. (c) Conclusion of cell death assay at 12 hours. Data shown as mean of triplicates ± SD of fluorescence intensity of Sytox. 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Journal of Immunology Research

fig3

Figure 3

Figure 3: Intracellular pH Regulates TRAIL-Induced Apoptosis and Necroptosis in Endothelial Cells 