Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes?

Table 4

Comparison of direct and sandwich IgG subclass assays.

		IgG1 beads	IgG2 beads	IgG3 beads	IgG4 beads	IgM beads
Sandwich assay	IgG1; HP6001	100%	14%	41%	15%	2%
	IgG2; HP6002	8%	100%	27%	25%	2%
	IgG3; HP6047	1%	0%	100%	4%	0%
	IgG4; HP6023	1%	1%	5%	100%	0%
Direct assay	IgG1; 4E3	100%	228%	260%	5%	1%
	IgG1; HP6001	100%	2%	88%	3%	1%
	IgG2; HP6002	5%	100%	105%	126%	6%
	IgG2; 31-7-4	14%	100%	245%	185%	2%
	IgG3; HP6050	0%	0%	100%	1%	0%
	IgG4; HP6023	0%	0%	2%	100%	0%