Regulatory T Cells and Their Prognostic Relevance in Hematologic Malignancies

Table 6

Most relevant published studies investigating the frequency and the prognostic significance of Tregs in Ph1-negative chronic myeloproliferative neoplasms.

Reference	Patients/controls evaluated	Samples tested	Marker panel used in Treg evaluation by flow cytometry	Treg frequency	Functional studies	Impact on prognosis
Hasselbalch et al. [66]	PV; ET; PMF; Controls	PBMC	CD4/CD25/CD127	Not increased	Inhibitory activity preserved	Marked expansion of Tregs in patients treated with IFN-α2 with than treated with hydroxyurea
Kovacsovics-Bankowski et al. [67]	PV; ET	PB	CD4/CD25/FoxP3/Ki-67	Not reported	Not performed	Tregs (including highly suppressive CD39^+ HLA-DR^+) increase in patients treated with PegINFα
Massa et al. [68, 69]	PMF	PB	CD4/CD25/CD127/FoxP3	Reduced	Not performed	In patients with CALR mutation genotype association with longer disease duration and hemoglobin concentration

PV: polycythemia vera; ET: essential thrombocythemia; PMF: primary myelofibrosis; PB: peripheral blood; PBMC: peripheral blood mononuclear cells; CALR: calreticulin; IFN: interferon.