Table 1: Diet composition of vitamin B6 deficient mouse model.

Diet compositionMineral/Kg dietVitamin/Kg diet

Sugar40%MgCl·6H2O17 gVitamin A14000 IU
Casein20%KCl5 gVitamin D1500 IU
Starch18%NaCl6 gVitamin E1200 IU
α-Cellulose8%FeCl3579 mgVitamin B113 mg
Oil5%MnCl4·4H2O270 mgVitamin B212 mg
Mineral3.5%CuSO4·5H2O40 mgVitamin B60/12/120 
Vitamin1%ZnCl263 mgFolic acid6.0 mg
Methionine0.30%Sn0.2 mgNiacin45 mg
Choline Bitartrate0.20%Pantothenic acid17 mg
Biotin0.10 mg
Vitamin K3.0 mg

Vitamin B6 was given as pyridoxine (PN) and its content was different in different diets. The diet was strictly defined as Non-VB6 Diet, Normal-VB6 Diet, and Excessive-VB6 Diet, which contained 0, 12 mg, and 120 mg of vitamin B6 per kg diet, respectively. The Excessive-VB6 Diet was mixed with 10 times vitamin B6 of the daily recommended intake.