Sirtuin 2 Regulates Microvascular Inflammation during Sepsis
E-selectin expression in WT, SIRT2KO, and SIRT2KI mice. We stained small intestinal tissue for E-selectin expression E-selectin (Cy3: red), Von Willebrand factor (VWF, FITC: green), and nuclear stain (DAPI: blue). (a) shows representative images of E-selectin expression in WT, SIRT2KO, and SIRT2KI mice with sham surgery while (b) shows fluorescence quantification of immunohistochemistry of three different mice in each group. There was no significant difference in E-selectin expression of WT, SIRT2KO, and SIRT2KI mice with sham surgery from each other. As shown in (c) (representative images) and (d) (fluorescence quantification from immunohistochemistry; in each group), the E-selectin expression increased in SIRT2KO sepsis mice versus that in WT sepsis mice while it was decreased in SIRT2KI sepsis mice versus that in SIRT2KO sepsis mice. versus that in WT sepsis group; # versus that in SIRT2KO sepsis group; using Tukey’s post hoc analysis; error bars: sem.