Sirtuin 2 Regulates Microvascular Inflammation during Sepsis
ICAM-1 expression in WT, SIRT2KO, and SIRT2KI mice. We stained small intestinal tissue for ICAM-1 expression. ICAM-1 (Cy3: red), Von Willebrand factor (VWF, FITC: green), and nuclear stain (DAPI: blue). (a) shows representative images of ICAM-1 expression in WT, SIRT2KO, and SIRT2KI mice with sham surgery while (b) shows fluorescence quantification of immunohistochemistry of three different mice in each group. There was no significant difference in ICAM-1 expression of WT, SIRT2KO, and SIRT2KI mice with sham surgery from each other. As shown in (c) (representative images) and (d) (fluorescence quantification from immunohistochemistry; in each group), the ICAM-1 expression increased in SIRT2KO sepsis mice versus that in WT sepsis mice while it was decreased in SIRT2KI sepsis mice versus that in WT and SIRT2KO sepsis mice. versus that in WT sepsis group; # versus that in SIRT2KO sepsis group; using Tukey’s post hoc analysis; error bars: sem.