The Immunogenicity of HLA Class II Mismatches: The Predicted Presentation of Nonself Allo-HLA-Derived Peptide by the HLA-DR Phenotype of the Recipient Is Associated with the Formation of DSA

Table 4

Recipient’s HLA-DR predicted presentation of self and nonself allo-HLA-derived peptide with highest affinity.

Recipient	Donor mismatches
	HLA-DRB1												HLA-DQB1												HLA-DQA1
TxID 13	DRB11:04
	Self			Nonself									Self			Nonself									Self			Nonself
	AA	IC50	% rank	AA	IC50	% rank							AA	IC50	% rank	AA	IC50	% rank							AA	IC50	% rank	AA	IC50	% rank
DRB04:05	158	1344	0.3	47	6022	7							158	2053	0.7	11	3057	1.8							146	3369	2.5	153	1032	0.15
DRB14:01	158	2007	0.9	47	3918	3.5							87	1426	0.5	24	1203	0.4							66	2605	1.5	69	1813	0.7
TxID 18	DRB07:01
	Self			Nonself									Self			Nonself									Self			Nonself
DRB11:01	158	938	1.8	6	1440	3							87	600	1.1	24	715	1.3							63	1465	3	45	349	0.5
DRB16:01	158	3272	3.5	6	2455	2.5							122	1910	1.6	47	1819	1.5							148	1063	0.7	45	1060	0.7
TxID 21
	Self			Nonself									Self			Nonself			Self			Nonself			Self			Nonself
DRB07:01	158	2199	2.5	16	1167	0.8							87	1908	1.9	8	1286	1	87	1908	1.9	23	1532	1.3	148	2180	2.5	54	2297	3
TxID 22	DRB07:01						DRB15:01
	Self			Nonself			Self			Nonself			Self			Nonself			Self			Nonself			Self			Nonself
DRB11:01	158	938	1.8	6	1440	3	47	919	1.8	88	1952	4.5	126	1848	4	87	600	1.1	126	1848	4	87	109	0.08	148	1774	4	45	349	0.5
DRB14:07	158	3491	1.2	31	7990	9	158	3491	1.2	86	6554	5.5	16	6760	6	83	2957	0.8	16	6760	6	87	1388	0.09	140	5765	4.5	69	2922	0.8
TxID 33							DRB13:01												DQB06:04
	Self			Nonself			Self			Nonself			Self			Nonself			Self			Nonself			Self			Nonself
DRB11:01	158	938	1.8	6	1440	3	47	919	1.8	24	526	0.9	87	600	1.1	24	715	1.3	87	166	0.17	24	275	0.4	63	1465	3	45	349	0.5
DRB16:01	158	3272	3.5	6	2455	2.5	47	2464	2.5	24	2196	1.9	122	1910	1.6	47	1819	1.5	87	710	0.4	24	1721	1.4	148	1063	0.7	45	1060	0.7
TxID 35							DRB15:01												DQB06:03
	Self			Nonself			Self			Nonself			Self			Nonself			Self			Nonself			Self			Nonself
DRB04:03	157	1784	0.03	37	9997	7	158	1784	0.03	67	13081	14	158	4464	0.7	47	7447	3.5	158	4464	0.7	87	7639	3.5	153	5451	1.3	69	6218	1.9
DRB13:02	159	3757	1.8	30	5424	4	159	3757	1.8	67	3244	1.3	16	4547	2.5	24	4083	2	16	4547	2.5	87	6480	5	3	1055	0.15	69	431	0.01

Note. Bold and underlined mismatches led to the production of DSA. Bold amino acid position, IC50, and percentile rank are for the peptides with highest affinity for the recipient’s HLA-DR antigens.
AA: amino acid position; % rank: percentile rank.