B-CD8⁺ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody

Figure 1

Antibody response against P3 mAb is abrogated in BALB/Xid mice. BALB/c (a) and BALB/Xid (b) mice were immunized subcutaneously with four doses of P3 mAb in PBS every two weeks. Serum samples were taken before the first immunization (day 0) and after the fourth dose (day 49). The kinetic of the response was determined by ELISA, on plates coated with P3 mAb, using alkaline phosphatase-conjugated goat anti-mouse IgG (Fcγ-specific). Sera were diluted to 1 : 100. The graphs show means ± SD of the values obtained in triplicate for each individual mouse.

(a)

(b)