B-CD8⁺ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody

Figure 2

P3 mAb has the capacity to activate in vivo B-1a cells. BALB/c peritoneal B-1a cells were collected 72 h after the immunization with a single dose of 50 μg of P3 mAb. (a) B-1a cell (B220⁺CD5⁺) gating strategy. (b) The ex vivo expression of CD25, CD69, and CD86 was evaluated by flow cytometry. Contour graph shows the results obtained for a representative mouse. Columns represent means ± SD of the values obtained in triplicate, , Mann–Whitney U test.

(a)

(b)