Journal of Immunology Research / 2017 / Article / Fig 3

Research Article

B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody

Figure 3

BALB/Xid mice transferred with B-1a plus B-2 cells recover IgG anti-P3 response. (a) Contour graph shows the results of B-1a and B-2 cell purification. (b) 5 × 106 B-1a cells, 50 × 106 B-2 cells, or 5 × 106 B-1a cells plus 50 × 106 B-2 cells from naïve BALB/c mice were transferred to BALB/Xid mice peritoneum, and mice were immunized subcutaneously with four doses of P3 mAb in PBS every two weeks. Serum samples were taken before the first immunization (day 0) and after the fourth dose (day 49). The reactivity was determined by ELISA on plates coated with P3 mAb using alkaline phosphatase-conjugated goat anti-mouse IgG (Fcγ-specific). Sera were diluted to 1 : 100. Symbols represent the median of the values obtained in triplicate for each individual mouse, , Duncan test. Different letters indicate statistical difference between the groups.