In Vitro Immunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis

Figure 4

Frequencies of IFN-γ-producing cells from CL patients after SLA and DI4G stimulation. PBMCs from CL patients () were stimulated with SLA (5 μg/ml), in the absence and presence of compound (300 nM), for 20 h. Cells were stained for CD3, CD4, CD8, CD56, and IFN-γ and collected using II FACSCanto flow cytometer. To analyze the data, the lymphocyte and NK populations were selected according to size and granularity and analyzed in CD3⁺ gate and CD3⁻ gate, respectively. Statistical analyses were performed using Wilcoxon t-test.