In Vitro Immunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis

Figure 5

Modulatory activity of DI4G in proliferation of PBMCs from CL patients. PBMCs from cutaneous leishmaniasis patients () were cultured with SLA (5 μg/ml), in the absence and presence of HsPNP inhibitor (300 nM), for 6 days. CD4⁺ T cells (a) and CD8⁺ T cells (b) were stained for CD3, CD4, CD8, and Ki-67 and collected using II FACSCanto flow cytometer. To analyze the data, the lymphocyte populations were selected according to size and granularity and analyzed in CD3⁺ gate. Statistical analyses were performed using Wilcoxon t-test.

(a)

(b)