Identification of Palmitoleic Acid Controlled by mTOR Signaling as a Biomarker of Polymyositis

Figure 2

Palmitoleic acid level is positively regulated by mTORC1 signaling. (a) LC-MS results showed the reduction of palmitoleic acid level in both rapamycin (rapa) and Torin1 treated PBMC. Results are averages of five independent experiments. Data represent mean ± SEM. and . (b-c) Western blots and quantification showed that mTORC1 activity, indicated by pp70S6K1 and p4EBP1, was dramatically decreased by both rapamycin (rapa) and Torin1 treated cells. Results are averages of three independent experiments. Data represent mean ± SEM. and . (d) LC-MS results showed the increasing of palmitoleic acid level in TSC1 knockdown (KD) cells. Results are averages of five independent experiments. Data represent mean ± SEM. . (e and f) Western blots and quantification showed that mTORC1 activity was dramatically increased by TSC1 KD. Results are averages of three independent experiments. Data represent mean ± SEM. and .

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