HLA Epitopes: The Targets of Monoclonal and Alloantibodies Defined

Figure 9

Epitope 5007 shared by the HLA class I A-locus antigens A31 and A33 and defined by isolucine (I) at position 73. The epitope is accessible on the dissociated antigens and show stronger reactivity when the peptide has been dissociated from the heavy chain. Position 73 is not exposed in an intact HLA class I antigen. After acid buffer treatment and neutralization of the eluate, epitope 5007 becomes exposed and reacts with the antibody 10-fold.